Composite
Part:BBa_K2305001
Designed by: PENG ZHAO Group: iGEM17_BIT (2017-10-19)
cycle amplification system and GFP/RFP dual fluorescent system
Pcat promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.
DNA Sequencing
We calculated the new part’s base number to be 1007bp. The figure1 is the result of DNA Sequencing. We can make a preliminary judgment that the sequences are correct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1525
Illegal BglII site found at 2704 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4385
Illegal AgeI site found at 4497 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3383
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Categories
Parameters
None |